Studies on Dengue Virus Infection in the Mosquitoes of Taiwan Rock Monkey Habitats at Gushan, Kaohsiung

T.H. Lin

1993 Vol.9 NO.11

Correspondence Author:


Form Apr. 1992 to Jun. 1993, forest mosquitoes, larvae and eggs were collected by sweep-net and ovitraps once a month in the area of Taiwan rock monkey habitats at Gushan, Kaohsiung, and were processed for isolation of dengue viruses. Collected adults were kept alive at least 7 days and then triturated for virus isolation through use of Aedes albopictus cells C6/36 and also through inoculation of Toxorhynchites ainboinensis mosquitoes. The inoculated cell cultures were kept at 32℃ for 7 days, and the inoculated mosquitoes were kept at 28±1℃ for 14 days. The cultures or mosquito head squash were examined for viruses with a fluorescent antibody technique. A total of 77 pools consisting of 2,670 female and 915 male mosquitoes were processed for virus isolation. The processed specimens were composed of 10 species of mosquitoes in 4 genera. No viruses were detected. It was suggested that the viruses didn’t spread 1 kilometer away from downtown to forest area, although the Kaohsiung City had been outbroken dengue fever many times.