Studies on Dengue Virus Infection in the Mosquitoes of Taiwan Rock Monkey Habitats at Gushan, Kaohsiung
1993 Vol.9 NO.11
Form Apr. 1992 to Jun. 1993, forest mosquitoes, larvae and eggs were collected by sweep-net and ovitraps once a month in the area of Taiwan rock monkey habitats at Gushan, Kaohsiung, and were processed for isolation of dengue viruses. Collected adults were kept alive at least 7 days and then triturated for virus isolation through use of Aedes albopictus cells C6/36 and also through inoculation of Toxorhynchites ainboinensis mosquitoes. The inoculated cell cultures were kept at 32℃ for 7 days, and the inoculated mosquitoes were kept at 28±1℃ for 14 days. The cultures or mosquito head squash were examined for viruses with a fluorescent antibody technique. A total of 77 pools consisting of 2,670 female and 915 male mosquitoes were processed for virus isolation. The processed specimens were composed of 10 species of mosquitoes in 4 genera. No viruses were detected. It was suggested that the viruses didn’t spread 1 kilometer away from downtown to forest area, although the Kaohsiung City had been outbroken dengue fever many times.