Establishment of Quality Control Method for Identifying Protobothrops mucrosquamatus and Viridovipera stejnegeri from Taiwan

DOI: 10.6525/TEB.20161220.32(24).002

Hui-Ching Yang, Cheng-Dow Lee

2016 Vol.32 NO.24

Correspondence Author: Hui-Ching Yang

  • Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Ministry of Health and Welfare, Taiwan


Incidence of snakebite envenomings remain high in the world. Nowadays it is an important and particular public health issue in the tropical and subtropical countries, and antivenom is the most effective treatment for snake bites at present. Venom is raw material for manufacturing antisera. Because of the stringent progress of the global pharmaceutical regulations and regulatory requirements, it is necessary to establish a set of more advanced quality control methods for identifying venom at its species. The goal in this research is to analyze venoms by high performance liquid chromatography (HPLC) and double immunodiffusion, and to determine the specific peaks for distinguishing the species in the chromatogram. A long-term analysis of wild snake venom would be beneficial to visualize if the changes in venom protein composition due to intraspecies variation of the geographical distribution or exotic species.

Keywords:Snakebite envenoming, Venom, Antivenom, High performance liquid chromatography (HPLC), HPLC chromatogram library